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TU Wien develops technique to replace tissue using 3D printing

The high-resolution 3D printing process is used to create tiny, porous spheres made of biocompatible and degradable plastic, which are then colonized with cells

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According to the Vienna University of Technology (TU Wien), researchers have created a novel 3D printing technique that enables the lab production of replacement tissue to replace injured cartilage, for example.

In this technique, a high-resolution 3D printing process is used to create tiny, porous spheres made of biocompatible and degradable plastic, which are then colonized with cells. These spheroids can then be arranged in any geometry, and the cells of the different units combine seamlessly to form a uniform, living tissue. Cartilage tissue, with which the concept has now been demonstrated at TU Wien, was previously considered particularly challenging in this respect.

Tiny spherical cages

“Cultivating cartilage cells from stem cells is not the biggest challenge. The main problem is that you usually have little control over the shape of the resulting tissue,” said Oliver Kopinski-Grünwald from the Institute of Materials Science and Technology at TU Wien, one of the authors of the current study. “This is also due to the fact that such stem cell clumps change their shape over time and often shrink.”

To prevent this, the research team at TU Wien is working with specially developed laser-based high-resolution 3D printing systems to create tiny cage-like structures that look like mini footballs and have a diameter of just a third of a millimeter. These serve as a support structure and form compact building blocks that can then be assembled into any shape.

TU Wien develops technique to replace tissue using 3D printing. The high-resolution process is used to create tiny, porous spheres.

Stem cells are first introduced into these football-shaped mini-cages, which quickly fill the tiny volume. “In this way, we can reliably produce tissue elements in which the cells are evenly distributed and the cell density is very high. This would not have been possible with previous approaches,” said Prof. Aleksandr Ovsianikov, head of the 3D Printing and Biofabrication research group at TU Wien.

Growing together

The team used differentiated stem cells – i.e. stem cells that can no longer develop into any type of tissue, but are already predetermined to form a specific type of tissue, in this case cartilage tissue. Such cells are particularly interesting for medical applications, but the construction of larger tissue is challenging when it comes to cartilage cells. In cartilage tissue, the cells form a very pronounced extracellular matrix – a mesh-like structure between the cells that often prevents different cell spheroids from growing together in the desired way.

If the 3D printed porous spheroids are colonized with cells in the desired way, the spheroids can be arranged in any desired shape. The research has shown that the cells of different spheroids also combine to form a uniform, homogeneous tissue.

“This is exactly what we have now been able to show for the first time,” said Kopinski-Grünwald. “Under the microscope, you can see very clearly: neighboring spheroids grow together, the cells migrate from one spheroid to the other and vice versa, they connect seamlessly and result in a closed structure without any cavities – in contrast to other methods that have been used so far, in which visible interfaces remain between neighboring cell clumps.”

TU Wien develops technique to replace tissue using 3D printing. The high-resolution process is used to create tiny, porous spheres.

The tiny 3D printed scaffolds give the overall structure mechanical stability while the tissue continues to mature. Over a few months, the plastic structures degrade – leaving behind the finished tissue in the desired shape.

Medical application

In principle, the new approach is not limited to cartilage tissue, but could also be used to tailor different kinds of larger tissues such as bone tissue. However, there is still work to be done to get to this stage – considering that, unlike in cartilage tissue, blood vessels would also have to be incorporated for these tissues above a certain size.

“An initial goal would be to produce small, tailor-made pieces of cartilage tissue that can be inserted into existing cartilage material after an injury,” said Oliver Kopinski-Grünwald. “In any case, we have now been able to show that our method for producing cartilage tissue using spherical micro-scaffolds works in principle and has decisive advantages over other technologies.”

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