Programmable living materials produced using 3D printing

Harnessing cells to make new types of materials that can grow, repair, and even respond to their environment

Stay up to date with everything that is happening in the wonderful world of AM via our LinkedIn community.

According to ACS, scientists are harnessing cells to make new types of materials that can grow, repair, and even respond to their environment. These solid ‘engineered living materials’ are made by embedding cells in an inanimate matrix formed in a desired shape. The researchers have reported that they have 3D printed a bioink containing plant cells that were then genetically modified – producing programmable materials. Applications could someday include biomanufacturing and sustainable construction.

Recently, researchers have been developing engineered living materials, primarily relying on bacterial and fungal cells as the live components. The unique features of plant cells have stirred enthusiasm for their use in engineered plant living materials (EPLMs). However, the plant cell-based materials created to date have had fairly simple structures and limited functionality. Ziyi Yu, Zhengao Di, and colleagues wanted to change that by making intricately shaped EPLMs containing genetically engineered plant cells with customizable behaviors and capabilities.

Programmable living materials produced using 3D printing. Harnessing cells to make new types of materials that can grow and repair.
After 24 days, the colors produced by plant cells in two different bioinks printed in this leaf-shaped engineered living material are clearly visible. Source: ACS.

The researchers mixed tobacco plant cells with gelatin and hydrogel microparticles that contained Agrobacterium tumefaciens, a bacterium commonly used to transfer DNA segments into plant genomes. This bioink mixture was then 3D printed on a flat plate or inside a container filled with another gel to form shapes such as grids, snowflakes, leaves, and spirals. Next, the hydrogel in the printed materials was cured with blue light – hardening the structures. During the ensuing 48 hours, the bacteria in the EPLMs transferred DNA to the growing tobacco cells. The materials were then washed with antibiotics to kill the bacteria. In the following weeks, as the plant cells grew and replicated in the EPLMs, they began producing proteins dictated by the transferred DNA.

In this proof-of-concept study, the transferred DNA enabled the tobacco plant cells to produce green fluorescent proteins or betalains – red or yellow plant pigments that are valued as natural colorants and dietary supplements. By printing a leaf-shaped EPLM with two different bioinks – one that created red pigment along the veins and the other a yellow pigment in the rest of the leaf – the researchers showed that their technique could produce complex, spatially controlled, and multifunctional structures. Such EPLMs, which combine the traits of living organisms with the stability and durability of non-living substances, could find use as cellular factories to churn out plant metabolites or pharmaceutical proteins, or even in sustainable construction applications, according to the researchers.

The research was funded by the National Key Research and Development Program of China, the National Natural Science Foundation of China, the Natural Science Foundation of Jiangsu Province, and the State Key Laboratory of Materials-Oriented Chemical Engineering.

Composites AM 2024

746 composites AM companies individually surveyed and studied. Core composites AM market generated over $785 million in 2023. Market expected to grow to $7.8 billion by 2033 at 25.8% CAGR. This new...

Edward Wakefield

Edward is a freelance writer and additive manufacturing enthusiast looking to make AM more accessible and understandable.

Related Articles

Leave a Reply

Your email address will not be published. Required fields are marked *

Back to top button
Close Popup
Privacy Settings saved!
Privacy Settings

When you visit any web site, it may store or retrieve information on your browser, mostly in the form of cookies. Control your personal Cookie Services here.

These cookies are necessary for the website to function and cannot be switched off in our systems.

Technical Cookies
In order to use this website we use the following technically required cookies
  • wordpress_test_cookie
  • wordpress_logged_in_
  • wordpress_sec

Decline all Services
Accept all Services


Join our 12,000+ Professional community and get weekly AM industry insights straight to your inbox. Our editor-curated newsletter equips executives, engineers, and end-users with crucial updates, helping you stay ahead.